Journal: Oncology reports

Article Title: PLCε knockdown enhances the radiosensitivity of castration‑resistant prostate cancer via the AR/PARP1/DNA‑PKcs axis.

doi: 10.3892/or.2020.7520

Figure Lengend Snippet: Figure 3. Continued. PLCε regulates DNA damage repair by altering the AR/DNA‑PKcs pathway. (C) mRNA and (D) protein expression levels of PLCε, AR, DNA‑PKcs and H2AX in PCa cells. ‘#’ indicates irradiated cells. *P<0.05, **P<0.01 and ***P<0.001. PCa, prostate cancer; PLCε, phospholipase Cε; AR, androgen receptor.

Article Snippet: Following blocking with 5% skimmed milk for 2 h at room temperature, the membranes were incubated with primary antibodies targeting PLCε (1:500; cat. no. sc-28402; Santa Cruz Biotechnology, Inc.), AR (1:1,000; cat. no. 5153; CST Biological Reagents Co., Ltd.), PARP1 (1:1,000; cat. no. ab32138; Abcam), DNA-dependent protein kinase catalytic subunit (DNA-PKcs; 1:1,000; cat. no. ab168854; Abcam), H2AX (1:1,000; cat. no. 7631; CST Biological Reagents Co., Ltd.), γ-H2AX (1:1,000; cat. no. 7631; CST Biological Reagents Co., Ltd.) and GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology) overnight at 4 ̊C, washed with TBS + 0.05% Tween‐20 (Beijing Solarbio Science & Technology Co., Ltd.), and subsequently incubated with a horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G secondary antibody (1:2,000; cat. no. TA130015; OriGene Technologies, Inc.) for 1 h at room temperature.

Techniques: Expressing, Irradiation

Journal: Oncology reports

Article Title: PLCε knockdown enhances the radiosensitivity of castration‑resistant prostate cancer via the AR/PARP1/DNA‑PKcs axis.

doi: 10.3892/or.2020.7520

Figure Lengend Snippet: Figure 7. PLCε knockdown inhibits the growth of CRPC in vivo. (A and B) Immunocompromised mice were subcutaneously injected with PLCε‑knockdown or control Bica‑R cells, and the tumor sizes and weights of the two groups are presented. (C) Comparison of positive rate of IHC in each group. (D) Expression of PLCε, AR, PARP1, DNA‑PKcs and γ‑H2AX in each group. Magnification, x200. *P<0.05 and ***P<0.001. PLCε, phospholipase Cε; CRPC, castration‑resistant prostate cancer; Bica‑R, Bicalutamide‑resistant; AR, Androgen receptor; PARP1, poly (ADP‑ribose) polymerase 1; H2AX, H2A histone family member X.

Article Snippet: Following blocking with 5% skimmed milk for 2 h at room temperature, the membranes were incubated with primary antibodies targeting PLCε (1:500; cat. no. sc-28402; Santa Cruz Biotechnology, Inc.), AR (1:1,000; cat. no. 5153; CST Biological Reagents Co., Ltd.), PARP1 (1:1,000; cat. no. ab32138; Abcam), DNA-dependent protein kinase catalytic subunit (DNA-PKcs; 1:1,000; cat. no. ab168854; Abcam), H2AX (1:1,000; cat. no. 7631; CST Biological Reagents Co., Ltd.), γ-H2AX (1:1,000; cat. no. 7631; CST Biological Reagents Co., Ltd.) and GAPDH (1:1,000; cat. no. 5174; Cell Signaling Technology) overnight at 4 ̊C, washed with TBS + 0.05% Tween‐20 (Beijing Solarbio Science & Technology Co., Ltd.), and subsequently incubated with a horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G secondary antibody (1:2,000; cat. no. TA130015; OriGene Technologies, Inc.) for 1 h at room temperature.

Techniques: Knockdown, In Vivo, Injection, Control, Comparison, Expressing

Journal: IEEE Transactions on Instrumentation and Measurement

Article Title: Group-Delay Measurement of Frequency-Converting Devices Using a Comb Generator

doi: 10.1109/tim.2010.2045972

Figure Lengend Snippet: Fig. 5. Block diagram of the DUT used in measurements. The mixer was a Minicircuits SRA-11+, the BPF is a Minicircuits PBP-70+, the amplifier is a Minicircuits MAR-2+, and the LO signal generator was an Agilent 8648B.

Article Snippet: For practical measurements, the DUT consisted of a passive mixer (Minicircuits SRA-11+) followed by a BPF (a Minicircuits PBP-70+) and an amplifier (Minicircuits MAR-2+), with additional components selected as per the application notes.

Techniques: Blocking Assay